HIV-1-infected individuals express higher levels of TLR2 and TLR4 mRNAs than healthy donors. TLR2 and TLR4 mRNA levels were measured using real-time PCR, and normalized with the housekeeping gene β-actin. Relative units of TLR2 and TLR4 transcripts versus β-actin transcripts are shown as a median and range. Comparisons were by Kruskal–Wallis ANOVA tests and Dunn's post-tests. The level of significance is p<0.05 (*); controls (n=21), −OI (HIV-1-infected patients without OIs, n=39), +OI (HIV-1-infected patients with OIs, n=10).