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. 2011 Nov 1;19(23):23327–23340. doi: 10.1364/OE.19.023327

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 6 — TNF-α detection performed on a PC using a sandwich ELISA assay. The antigen concentration decreases from left to right. (a) Label-free image of the PC surface showing the presence of capture antibody spots on the sensor. (b) Fluorescence detection at a single resonance angle of 10° after assay is completed. (c) Fluorescence detection using the masked detection for the same PC surface showing improved contrast, recognition and uniformly higher fluorescence output.