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. 2011 Jan 7;19(2):1016–1026. doi: 10.1364/OE.19.001016

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 4 — SLIM-fluorescence multimodal imaging. (a)-(b) Combined multimodal images of cultured neurons (19 DIV) acquired through SLIM (red) and fluorescence microscopy of anti-MAP2 stained soma and dendrites (green) and DAPI-stained nuclei (blue). (c) Optical path-length fluctuations along the dendrites (green) and axon (red) retrieved from the inset of (a). (d) Synaptic boutons of a mature hippocampal neuron (33 DIV) immunochemically labeled for synapsin (green) and MAP2 (blue). Scale bars: 20 μm.