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. 2011 Jan 7;19(2):1016–1026. doi: 10.1364/OE.19.001016

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©2011 Optical Society of America

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.

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Fig. 5 — ( Media 1^{(9.3MB, MOV)}) SLIM simulated DIC and Laplacian. Since the quantitative phase information is obtained, all other microscopy such as DIC, phase contrast and dark field can be numerically simulated through SLIM imaging. Objective: Zeiss EC Plan-Neofluar 40 × /0.75. (a) SLIM imaging of glial-microglia cell culture. (b) SLIM imaging with ROI (region of interest) enhancement. (c) Simulated DIC-based on the phase measurement of SLIM. (d) Laplacian of SLIM image with ROI enhancement. Scale bars: 10 μm.