Abstract
Three fluorescein-labeled lectins have been shown to exhibit specificity for the surface of cells in different layers of the epidermis in the newborn rat. An isolectin from seeds of Bandeiraea simplicifolia with specificity for alpha-D-galactosyl end groups labeled the basal and lower spinous cells; a lectin from Ulex europaeus exhibiting specificity for alpha-L-fucosyl units outlines the surface of spinous cells, and a second lectin from B. simplicifolia, with specificity for N-acetyl-D-glucosamine, labels the cornified cells. Appropriate blocking experiments have confirmed the specific nature of the binding.
Full text
PDF


Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dabelsteen E., Fejerskov O., Norén O., Mackenzie I. C. Concanavalin A and ricinus communis receptor sites in normal human oral mucosa. J Invest Dermatol. 1978 Jan;70(1):11–15. doi: 10.1111/1523-1747.ep12543358. [DOI] [PubMed] [Google Scholar]
- Dabelsteen E., Mackenzie I. C. Expression of Ricinus communis receptors on epithelial cells in oral carcinomas and oral wounds. Cancer Res. 1978 Dec;38(12):4676–4680. [PubMed] [Google Scholar]
- Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
- Essner E., Schreiber J., Griewski R. A. Localization of carbohydrate components in rat colon with fluoresceinated lectins. J Histochem Cytochem. 1978 Jun;26(6):452–458. doi: 10.1177/26.6.670681. [DOI] [PubMed] [Google Scholar]
- Etzler M. E., Branstrator M. L. Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins. J Cell Biol. 1974 Aug;62(2):329–343. doi: 10.1083/jcb.62.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gahmberg C. G., Hakomori S. I. Altered growth behavior of malignant cells associated with changes in externally labeled glycoprotein and glycolipid. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3329–3333. doi: 10.1073/pnas.70.12.3329. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldman R. Effect of Concanavalin A on phagocytosis by macrophages. FEBS Lett. 1974 Sep 15;46(1):209–213. doi: 10.1016/0014-5793(74)80370-4. [DOI] [PubMed] [Google Scholar]
- Goldstein I. J., Hayes C. E. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem. 1978;35:127–340. doi: 10.1016/s0065-2318(08)60220-6. [DOI] [PubMed] [Google Scholar]
- Hashimoto K., King L. E., Jr, Yamanishi Y., Beachey E. H., Maeyens E. Identification of the substance binding pemphigus antibody and concanavalin A in the skin. J Invest Dermatol. 1974 Apr;62(4):423–435. doi: 10.1111/1523-1747.ep12701675. [DOI] [PubMed] [Google Scholar]
- Lennette D. A. An improved mounting medium for immunofluorescence microscopy. Am J Clin Pathol. 1978 Jun;69(6):647–648. doi: 10.1093/ajcp/69.6.647. [DOI] [PubMed] [Google Scholar]
- Lyer P. N., Wilkinson K. D., Goldstein L. J. An -N-acetyl-D-glycosamine binding lectin from Bandeiraea simplicifolia seeds. Arch Biochem Biophys. 1976 Nov;177(1):330–333. doi: 10.1016/0003-9861(76)90444-6. [DOI] [PubMed] [Google Scholar]
- Murphy L. A., Goldstein I. J. Five alpha-D-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds. J Biol Chem. 1977 Jul 10;252(13):4739–4742. [PubMed] [Google Scholar]
- Nicolson G. L., Blaustein J. The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces. Biochim Biophys Acta. 1972 May 9;266(2):543–547. doi: 10.1016/0005-2736(72)90109-5. [DOI] [PubMed] [Google Scholar]
- Nicolson G. L., Yanagimachi R., Yanagimachi H. Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs. J Cell Biol. 1975 Aug;66(2):263–274. doi: 10.1083/jcb.66.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nieland M. L. Epidermal intercellular staining with fluorescein-conjugated phytohemagglutinins. J Invest Dermatol. 1973 Feb;60(2):61–66. doi: 10.1111/1523-1747.ep12724137. [DOI] [PubMed] [Google Scholar]
- Peters B. P., Goldstein I. J. The use of fluorescein-conjugated Bandeiraea simplicifolia B4-isolectin as a histochemical reagent for the detection of alpha-D-galactopyranosyl groups. Their occurrence in basement membranes. Exp Cell Res. 1979 May;120(2):321–334. doi: 10.1016/0014-4827(79)90392-6. [DOI] [PubMed] [Google Scholar]
- Sharon N., Lis H. Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972 Sep 15;177(4053):949–959. doi: 10.1126/science.177.4053.949. [DOI] [PubMed] [Google Scholar]
- Skutelsky E., Farquhar M. G. Variations in distribution of con A receptor sites and anionic groups during red blood cell differentiation in the rat. J Cell Biol. 1976 Oct;71(1):218–231. doi: 10.1083/jcb.71.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stegman S. J., Fukuyama K., Epstein W. L. Inhibition of the in vivo effects of concanavalin-A on mammalian epidermis by alpha-methyl-D-glucopyranoside. J Invest Dermatol. 1976 Jan;66(1):17–21. doi: 10.1111/1523-1747.ep12478012. [DOI] [PubMed] [Google Scholar]
- Van Lis J. M., Kalsbeek G. L. The interaction of Concanavalin A and the surface coat of stratified squamous epithelium. Br J Dermatol. 1975 Jan;92(1):27–35. doi: 10.1111/j.1365-2133.1975.tb03029.x. [DOI] [PubMed] [Google Scholar]
- Winand R., Luzzati D. Cell surface changes during myoblast differentiation: preparation and carbohydrate composition of plasma membranes. Biochimie. 1975;57(6-7):764–771. [PubMed] [Google Scholar]
- Yahara I., Edelman G. M. Modulation of lymphocyte receptor mobility by locally bound concanavalin A. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1579–1583. doi: 10.1073/pnas.72.4.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]






