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. Author manuscript; available in PMC: 2012 Oct 29.
Published in final edited form as: Crit Care Clin. 2011 Jul;27(3):705–718. doi: 10.1016/j.ccc.2011.04.002

Figure 3. Electroporation-mediated gene transfer of subunits of the Na+,K+-ATPase can both protect from subsequent LPS-induced lung injury and reverse pre-existing LPS-induced lung injury.

Figure 3

A. Protection Studies. One hundred μg of plasmid in 50 μl were administered intratracheally to mice by electroporation. One day later, LPS (4 mg/kg) was administered to the lungs and 24 or 27 hours after this, lungs were removed for wet-to-dry ratio analysis (mean ± sem; n=5). B. Treatment Studies. LPS (4 mg/kg) was administered intratracheally to Balb/c mice and one day later, 100 μg of plasmid was delivered to the lungs by electroporation. Twenty-four or forty-eight hours later, lungs were removed for gravimetric analysis. Wet-to-dry ratios are shown as mean ± SEM (n=5). Statistical analysis was by non-parametric Mann-Whitney U test. Taken with permission from ref ,51.