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. Author manuscript; available in PMC: 2012 Oct 29.
Published in final edited form as: Gene Ther. 2010 Jan 7;17(4):541–549. doi: 10.1038/gt.2009.166

Figure 2. SP-C promoter plasmids localize to the nuclei of alveolar type II epithelial cell lines but not of other cell types.

Figure 2

Different cell lines were cytoplasmically injected with equal numbers of plasmids carrying the human SP-C promoter and the location of the DNA was detected by in situ hybridization eight hours later. A. Representative cells displaying pCR-SPC nuclear or cytoplasmic localization. B. The percentage of cells showing greater than 90% nuclear signal was quantified for each cell type. The pCRII-ΔDTS plasmid is included as a negative control for background nuclear import. Data are plotted as mean ± st. dev. from three or more independent experiments. Approximately 100 cells per experiment were imaged for each cell type.