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. Author manuscript; available in PMC: 2012 Oct 29.
Published in final edited form as: Gene Ther. 2010 Jan 7;17(4):541–549. doi: 10.1038/gt.2009.166

Figure 4. Nuclear import of the SP-C plasmid in MLE-12 cells is abrogated following siRNA knockdown of TTF-1, NFIA, or GATA-6.

Figure 4

MLE-12 cells were treated with siRNAs against the transcription factors TTF-1, NFIA, and GATA-6, or a non-targeted siRNA as a negative control. A. Western blots were performed on cell extracts at the indicated times following siRNA transfection. B. Forty-eight (TTF-1) or 72 (NF1 and GATA-6) hours post-transfection, pCR-SPC was microinjected into the cytoplasm of the cells and its cellular localization was determined 8 hours later by situ hybridization. The pCRII-ΔDTS plasmid is included as a negative control for background nuclear import. Data are plotted as mean ± st. dev. from three or more independent experiments. (*) indicates p<0.05 when compared to the non-targeting control.