Figure 6.

pUC inhibits the FAK migratory signaling pathway and inhibition of ERK can further influence the FAK signal. A, Cell lysates of control, pSV-transfected, and pUC-transfected U251 and 5310 non-GICs and GICs with and without irradiation were collected. Western blot analysis was performed for FAK migratory signaling molecules Rac-1, Cdc42, pCdc42/Rac-1, Ras, ERK, and pERK using their specific antibodies. GAPDH served as a loading control. B, The total protein lysates from U251 and 5310 non-GICs and GICs were collected from U0126 (10 μM) and DMSO treatments. SDS-PAGE was conducted as described in Materials and methods. Western blotting was performed to determine the protein expression levels of ERK, pERK, FAK, pFAK (Tyr³⁹⁷), pFAK (Tyr⁹²⁵), vinculin, α-actinin, Rac-1, Cdc42, pCdc42/Rac-1, and Ras using their specific antibodies.