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. 2012 Oct 29;2:149. doi: 10.3389/fonc.2012.00149

Figure 1.

Figure 1

Irradiated-CT26/PSA cells cause BM-DC maturation in the presence of rHSPs. BM-DCs (1.5 × 106 in 1 ml) were incubated with medium alone (control) or LPS (10 μg/ml) or were cocultured with irradiated-CT26/PSA cells (1.5 × 106 in 1 ml) in PBS or 10 μg/ml of BSA, rHsp70, or rHsp70C′-PSA. After 48 h, the cells were harvested and stained with antibodies against MHC class II molecule I-Ad and LAMP1. Column 1, DAPI nuclear staining; column 2, LAMP1 staining; column 3, MHC class II intracellular staining; column 4, merged images. Images were acquired using confocal microscopy at a magnification of ×400. This experiment was repeated three times with reproducible results. Scale bars, 20 μm.