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. 2012 Oct 29;2:149. doi: 10.3389/fonc.2012.00149

Figure 2.

Figure 2

Exogenous rHSPs enhance the expression of pro-inflammatory cytokines. BM-DCs (1.5 × 106 in 1 ml) were incubated with medium alone (control) or LPS (10 μg/ml) or were cocultured at 37°C for 24 h with irradiated-CT26/PSA cells (1.5 × 106 in 1 ml) of PBS or 10 μg/ml of BSA, rHsp70, or rHsp70C′-PSA in the absence or presence of 10 μg/ml of polymyxin B. The supernatants were then harvested and the concentration of IL-12 (A) or TNF-α (B) measured by ELISA. The data are the mean ± SD for three-independent experiments. *Indicates p < 0.05.