Figure 9.

Verification of microarray results via qRT-PCR. RNA from GAB-treated Arabidopsis wild-type seedlings (A,B) and gun4-1 mutant seedlings (C,D) was extracted 6 h (A,C) and 24 h (B,D), respectively, after light exposure. Transcript abundance of a defined set of genes (see Tables 2 and 3 and Table S2 in Supplementary Material) was determined by quantitative real-time PCR and calculated as relative expression [2^−ΔΔCt] compared to untreated Arabidopsis wild-type Col-0 seedlings. Relative expressions obtained by qRT-PCR are plotted against relative expressions in microarray experiments (sampling 6 h after light exposure) in order to compare relative expressions in both experimental approaches.