Table 2.
Listed are genes that showed a significantly (P ≤ 0.05) decreased transcript abundance after 6 h of de-etiolation in response to inhibited ALA formation (GAB) and in the gun4-1 mutant.
| AGI number | GAB vs. Col-0 fold change | GAB vs. Col-0 P-value | gun4-1 vs. Col-0 fold change | gun4-1 vs. Col-0 P-value | SUBA localization | Description | qRT-PCR performed (see Table S2 in Supplementary Material) |
|---|---|---|---|---|---|---|---|
| At5g08030 | 0.247 | 0.000 | 0.249 | 0.000 | Mito, Cp, ER, Nuc, Ex, Vac | Glycerophosphoryl diester phosphodiesterase | |
| At1g13650 | 0.273 | 0.000 | 0.381 | 0.000 | Mito, Perox | Unknown protein | |
| At2g20570 | 0.300 | 0.000 | 0.351 | 0.000 | Cyt | GPRI1 (GBF’S Pro-rich region-interacting factor 1) | Yes |
| At5g46690 | 0.345 | 0.000 | 0.344 | 0.000 | Nuc | Beta HLH protein 71 (bHLH071) | |
| At1g61800 | 0.347 | 0.000 | 0.486 | 0.000 | Cp, Mito, Ex, PM | Glucose-6-phosphate transmembrane transporter (GPT2) | Yes |
| At3g26960 | 0.372 | 0.000 | 0.288 | 0.000 | Ex, ER, Nuc | Unknown protein | Yes |
| At5g55450 | 0.376 | 0.005 | 0.278 | 0.000 | Ex, ER | Protease inhibitor | |
| At4g04610 | 0.385 | 0.019 | 0.166 | 0.000 | Cp | APS reductase 1 (APR1) | Yes |
| At4g28250 | 0.387 | 0.003 | 0.182 | 0.000 | Ex, ER, Vac | ATEXPB3 (Expansin B3) | Yes |
| At2g20750 | 0.407 | 0.000 | 0.176 | 0.000 | Ex | ATEXPB1 (Expansin B1) | |
| At3g61820 | 0.414 | 0.000 | 0.344 | 0.000 | Cp, Ex | Aspartyl protease family protein | Yes |
| At3g47380 | 0.431 | 0.005 | 0.465 | 0.002 | Ex, Mito, ER, Nuc | Invertase/pectin methylesterase inhibitor | |
| At1g56650 | 0.439 | 0.000 | 0.355 | 0.000 | Nuc | PAP1 (production of anthocyanin pigment 1); DNA binding/transcription factor | Yes |
| At5g45650 | 0.443 | 0.001 | 0.356 | 0.000 | Mito, ER, Ex | Subtilase family protein | Yes |
| At1g24020 | 0.457 | 0.017 | 0.160 | 0.000 | Cyt, Perox | MLP423 (MLP-like protein 423) | Yes |
| At3g59670 | 0.459 | 0.000 | 0.528 | 0.000 | Nuc | Unknown protein | |
| At2g31270 | 0.460 | 0.000 | 0.645 | 0.005 | Mito, Nuc, Cp | CDT1A (homolog of yeast CDT1 A); cyclin-dependent protein kinase | |
| At4g11460 | 0.468 | 0.014 | 0.271 | 0.000 | Ex, ER, Nuc, Vac | Protein kinase family protein | |
| At4g34588 | 0.474 | 0.003 | 0.428 | 0.000 | No data | CPuORF2 (conserved peptide upstream open reading frame 2) | |
| At4g34590 | 0.474 | 0.003 | 0.428 | 0.000 | Nuc | GBF6 (G-BOX BINDING FACTOR 6) | Yes |
| At5g05960 | 0.477 | 0.014 | 0.170 | 0.000 | ER, Ex | Protease inhibitor/seed storage/lipid transfer protein (LTP) family protein | |
| At4g28680 | 0.480 | 0.001 | 0.432 | 0.000 | Cyt, Mit, ER | Putative tyrosine decarboxylase | |
| At3g05600 | 0.481 | 0.005 | 0.459 | 0.001 | Cp, Perox, Cyt | Putative epoxide hydrolase | Yes |
| At4g39510 | 0.485 | 0.001 | 0.369 | 0.000 | Mito, ER, Cyt, Ex, Nuc | CYP96A12; electron carrier/heme binding/iron ion binding/monooxygenase/oxygen binding | Yes |
| At1g66940 | 0.493 | 0.002 | 0.265 | 0.000 | Ex, ER, Cyt, Ex | Protein kinase-related | Yes |
| At4g38950 | 0.500 | 0.004 | 0.393 | 0.000 | Nuc | Kinesin motor family protein | Yes |
In contrast to the results shown in Figure 5, there are only transcripts listed with a fold change ≤0.5. Given are the fold changes, corresponding P-values, the putative subcellular localizations using the SUBA database (Heazlewood et al., 2007) and gene annotations based on the TAIR9 release of the Arabidopsis genome. (Cp, chloroplasts; Cyt, cytosol; ER, endoplasmic reticulum; Ex, extracellular; Mito, mitochondria; Nuc, nucleus; Perox, peroxisome; PM, plasma membrane; Vac, vacuole).