Table 3.
Listed are genes that showed a significantly (P ≤ 0.05) increased transcript abundance after 6 h of de-etiolation in response to inhibited ALA formation (GAB) and in the gun4-1 mutant.
| AGI number | GAB vs. Col-0 fold change | GAB vs. Col-0 P-value | gun4-1 vs. Col-0 fold change | gun4-1 vs. Col-0 P-value | SUBA localization | Description | qRT-PCR performed (see Table S2 in Supplementary Material) |
|---|---|---|---|---|---|---|---|
| At1g05560 | 4.010 | 0.000 | 2.057 | 0.000 | Cp, Mito, Cyt | UDP-glucose:4-aminobenzoate acylglucosyltransferase (UGT75B1) | Yes |
| At1g22890 | 3.708 | 0.000 | 6.331 | 0.000 | ER, Nuc, Cp | Unknown protein | Yes |
| At2g04040 | 3.267 | 0.001 | 3.156 | 0.000 | PM, Ex, Vac | TX1; antiporter/multidrug efflux pump/multidrug transporter/transporter | |
| At3g22060 | 2.838 | 0.000 | 3.278 | 0.000 | Ex, Vac | Receptor protein kinase-related | Yes |
| At2g19800 | 2.838 | 0.000 | 2.686 | 0.000 | Cyt | MIOX2 (Myo-inositol oxygenase 2) | |
| At5g16980 | 2.773 | 0.000 | 1.635 | 0.011 | Cyt, Mito, Cp | Putative NADP-dependent oxidoreductase | |
| At3g20340 | 2.728 | 0.000 | 1.903 | 0.001 | Nuc | Down-regulated under photo-oxidative stress. | |
| At5g43450 | 2.722 | 0.000 | 3.317 | 0.000 | Cyt, Nuc | Putative 2-oxoglutarate-dependent dioxygenase | |
| At3g47340 | 2.680 | 0.000 | 5.921 | 0.000 | Cyt, Cp | ASN1 (glutamine-dependent asparagine synthase 1) | Yes |
| At1g05680 | 2.591 | 0.005 | 2.247 | 0.003 | Mito, Cyt, Cp | UDP-glucoronosyl/UDP-glucosyl transferase | Yes |
| At5g16970 | 2.590 | 0.000 | 1.791 | 0.000 | AT-AER (2-alkenal reductase) | ||
| At3g28740 | 2.455 | 0.001 | 2.472 | 0.000 | Mito, ER, Ex, PM | CYP81D1; electron carrier/heme binding/iron ion binding/monooxygenase/oxygen binding | Yes |
| At3g20270 | 2.445 | 0.011 | 2.036 | 0.012 | Cp, Cyt, Ex | Lipid-binding serum glycoprotein family protein | |
| At2g17880 | 2.423 | 0.001 | 3.176 | 0.000 | Cp, Mito, Nuc | Putative DNAJ heat shock protein | Yes |
| At1g35140 | 2.362 | 0.000 | 12.734 | 0.000 | Ex | Phosphate induced 1 (PHI-1) | |
| At4g31870 | 2.340 | 0.002 | 2.257 | 0.001 | Cp, Mito, Perox, Nuc | ATGPX7 (glutathione peroxidase 7) | Yes |
| At1g08630 | 2.307 | 0.001 | 8.617 | 0.000 | Cp, Nuc, Cyt | THA1 (threonine aldolase 1) | Yes |
| At5g57560 | 2.218 | 0.000 | 6.492 | 0.000 | Ex, Mito, Er | TCH4 (touch 4); hydrolase, acting on glycosyl bonds/xyloglucan:xyloglucosyl transferase | Yes |
| At5g06860 | 2.187 | 0.001 | 1.775 | 0.001 | Ex | PGIP1 (polygalacturonase inhibiting protein 1) | |
| At2g29340 | 2.143 | 0.001 | 2.036 | 0.000 | Cyt, Ex, ER | Short-chain dehydrogenase/reductase (SDR) family protein | Yes |
| At5g17300 | 2.069 | 0.001 | 2.115 | 0.000 | Nuc | Myb family transcription factor | Yes |
| At4g15260 | 2.044 | 0.000 | 2.059 | 0.000 | Cyt, Cp | UDP-glucoronosyl/UDP-glucosyl transferase | Yes |
| At5g20230 | 2.042 | 0.005 | 22.472 | 0.000 | PM | ATBCB (blue-copper binding protein) | Yes |
| At5g14470 | 2.010 | 0.001 | 6.425 | 0.000 | Cyt, Nuc | GHMP kinase-related | |
| At3g15760 | 2.005 | 0.001 | 2.027 | 0.000 | Ex, Mito, Nuc, Cp | Unknown protein | Yes |
In contrast to the results shown in Figure 5, there are only transcripts listed with a fold change ≥2.0. Given are the fold changes, corresponding P-values, the putative subcellular localizations using the SUBA database (Heazlewood et al., 2007) and gene annotations based on the 9.0 release of the Arabidopsis genome. (Cp, chloroplasts; Cyt, cytosol; ER, endoplasmic reticulum; Ex, extracellular; Mito, mitochondria; Nuc, nucleus; Perox, peroxisome; PM, plasma membrane; Vac, vacuole).