Figure 4. Effect of IFN-γ and IL-4 on the axonal growth within the nerve guidance scaffold.
(a) Longitudinal section of NF160 staining for axons. Scale bar = 1 mm (b) Merged magnified images with DAPI magnified images. Scale bar = 250 μm (c) Quantitative analysis of NF160 at the distal ends. Mean ± s.d. n = 4. ***P < 0.001 (Student's t-test). Asterisks denote a significant difference compared with non-cytokine treated control scaffold. Number of axons at the distal end of the IL-4 treated scaffold is ∼20 times higher in comparison to the non-treated control scaffold.