Figure 1.
(a) Real-time shifts in microring resonances accompanying target hybridization highlight that despite over an 80-fold increase in size, full length mRNA elicits a much lower sensor response than smaller miRNA molecules based on steric inhibition and diffusion limitations. (b) Small DNA chaperones were pre-hybridized to a 2.56 nM concentration of myc mRNA before being flowed across an array of ssDNA-presenting microring resonators. The chaperone/mRNA mixture was introduced to the sensor array at t=0 minutes, and the resultant responses demonstrate the enhanced rate of target binding in the presence of chaperones.