Figure 4.
The concentration-dependent sensor response for direct (a–c) and bead-enhanced (d–f) mRNA detection. In each trace sample or beads was injected at t=0 minutes. Direct hybridization responses (a–c) were quantitated at 60 minutes and bead-enhanced responses (d–f) at 45 minutes. These pseudo-equilibrium time points were as a balance between minimizing total assay time while still maintaining quantitation capabilities. Calibration plots (g–i) demonstrate the full 3–4 order of magnitude dynamic range achieved combining direct and bead-based detection.