Fig. 5.

Four-region, multi-parameter cell capture. A mixture of HuT 78, Ramos and Prostate cancer cells was continuously captured at 0.05 mL/h for 30 min, followed by a wash step (2 mL/h for 2.5 min). Fluorescence images were taken at each antibody coated area to calculate capture density. The purity of the anti-CD4 zone was 79 ± 14%, while the purity of the anti-CD19 zone was 100% ± 8%. The cell mixture concentration was 4600 ± 300 cells/μL; the mixture of the three cells was approximately 1:1.8:1 (Hut 78:Ramos:CRL-1435). Error bars represent the standard deviation of three different microscope fields of view in the same antibody coated region.