MSC surface marker analysis reveals limited change during cell passaging. Single color cell surface marker analysis of cells from PCBM1641 and PCBM1632 at P3, P5, and P7 with antibodies specific for CD29, CD44, CD166, CD90, CD73, CD105, and STRO-1. Unstained controls are shown as the white histogram; shaded histograms represent samples stained with surface maker antibodies. The marker in each panel shows the gates used to identify percent positive cells. MSC, multipotent stromal cells.