FIG. 4.

Adipogenic precursor frequency can differ between cell lines and with cell passaging. Limiting dilution was conducted as described in Materials and Methods. The fraction of responding wells is represented as a function of dose of PCBM1641 (A) or PCBM1632 (C) at P3, P5, and P7. (B and D) show plots of the fraction of nonresponding wells as a function of cell dose on a semi-log plot for PCBM1641 (B) or PCBM1632 (D) at P3, P5, and P7. The trend lines were used to calculate the frequency of adipogenic precursors. Error bars represent standard deviation; n=3. R² values were >0.96 for PCBM1641 and >0.85 for PCBM1632 at all passages.