FIG. 7.
MSCs of different sizes have different adipogenic precursor frequencies. Cell size thresholds were based on separate FSC sorting gates that were applied to the upper (large cells) and lower (small cells) thirds of an FSC histogram plot. The middle third was not analyzed. (A) The fraction of responding wells was plotted as a function of cell dose in sorted small and large cells from PCBM1641 following limiting dilution analysis. (B) A plot of nonresponding wells as a function of cell dose. The trend line was used to calculate precursor frequency in populations of small and large cells. Error bars represent standard deviation; n=3 for both cell populations. R2 values were 0.99 for small cells and 0.99 for large cells.