FIG. 8.

Differentiation induces differences in adipocyte-specific gene expression levels that vary between cell lines and passages. The relative fold change in RNA expression for (A) fatty acid binding protein 4 (FABP4), (B) CCAAT/enhancer binding protein alpha (C/EBPα), (C) peroxisome proliferator-activated receptor gamma (PPARγ), (D) Adipsin, (E) Adiponectin, (F) acyl-CoA synthetase short-chain family member 2 (ACSS2), and (G) lipoprotein lipase (LPL) was measured for PCBM1641 and PCBM1632 cells from P3, P5, and P7 21 days after induction of adipose-lineage differentiation. Black and white bars represent PCBM1641 and PCBM1632 respectively. The y-axis indicates fold change in gene expression, and is represented in thousands in FABP4, Adiponectin, and LPL. Error bars represent standard deviation; n=3 for all samples. (H) Statistical analysis summary of data represented in A–G. Analysis completed between donors and between P3, P5, and P7; S indicates significance at p<0.05, NS indicates nonsignificance at p>0.05.