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. 2012 Jun 14;18(11):877–889. doi: 10.1089/ten.tec.2011.0736

Table 1.

Forward and Reverse Primer Sequences for Adipocyte-Specific Genes Used in Quantitative Polymerase Chain Reaction

Gene name Forward sequence (5′–3′) Reverse sequence (5′–3′) Melting temperature (°C) Amplicon size (bp)
Adipsin ACGACCTCCTGCTGCTAC AGGAGTGGATGACTTCATTG 55 493
Adiponectin ATCTCCTCCTCACTTCCATT ATGTCTCCCTTAGGACCAAT 55 304
PPARg CCTGAAACTTCAAGAGTACCA TCATGAGGCTTATTGTAGAGC 55 96
C/EBPa GAAGTCGGTGGACAAGAAC CATTGTCACTGGTCAGCTC 55 140
LPL AGACACAGCTGAGGACACTT GCACCCAACTCTCATACATT 55 137
FABP4 ACTGCAGATGACAGGAAAGT CACCACCAGTTTATCATCCT 55 126
ACSS2 GCAGGGTAAACTGAAAGAGA GTGACGTAGGAATGACCAGT 55 315