Short-term effect of NAA on tobacco leaf-strip
curvature (A) and longer-term effect of FC on strip length (B). In A,
10 strips were each anchored by one end to small rubber blocks in a
Petri dish and incubated in control solution (○) (10 mm
Suc, 10 mm KCl, and 0.5 mm Mes/BTP [pH 6.0])
or the same solution also including 10 μm NAA (•), or
first in control solution for 2 h followed by NAA (▪). Images of
the strips were captured every 15 min and curvature was recorded
relative to curvature at time 0. In B, 18 strips per treatment were
incubated in the same control solution (○) or the same solution plus
FC (♦) for 20 h. At each time point they were gently removed
from solution, their lengths measured, and then returned to the
incubation solution. Error bars indicate 95% confidence limits for
mean change (Student's t test) from time 0.