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. 2012 Oct 29;199(3):481–496. doi: 10.1083/jcb.201201133

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© 2012 Dornier et al.

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Figure 6. — Endogeneous TspanC8 tetraspanins are required for ADAM10 exit from the ER. (A) Analysis of Tspan5, Tspan14, or Tspan15 expression using RT-qPCR 24 h after the initiation of Tspan14 or Tspan15 silencing in HCT116 and PC3 cells, respectively. The figure shows the mean ± SD of three independent experiments in triplicate. (B) Flow cytometric analysis of the surface expression of ADAM10 in HCT116 or PC3 cells 2 d after the initiation of Tspan14 or Tspan15 silencing, respectively. (C) Confocal microscope analysis of the distribution of ADAM10 (red) and PDI (green) in Triton X-100 permeabilized HCT116 or PC3 cells after 2 d of silencing with a control siRNA or siRNA targeting Tspan14 (HCT116) or Tspan15 (PC3). Bar, 10 µm. All experiments were performed at least twice with similar outcome.