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. 2012 Oct 29;199(3):497–512. doi: 10.1083/jcb.201201091

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© 2012 Chen et al.

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Figure 5. — Effects of initial conformations of α_Lβ₂ and subsequent changes thereof on ligand dissociation under force. (A and B) Short (<10 s) lifetime means (A) and long (>10 s) lifetime fractions (B) of α_Lβ₂–ICAM-1 bonds without switching (t_tot = t₁; ◇) versus force (f₀ = f₁; data obtained from Chen et al., 2010). These were further segregated into two groups, based on whether the molecular stiffness k_mol was greater or less than 0.5 pN/nm. k_mol was measured from the position-ramp phase using the stretch method shown in Fig. S2 A. Error bars represent SEM of >40 measurements. (C and D) Dependence on postswitch force (f₁) of total (t_tot) short (<10 s) lifetime mean (C) and long (>10 s) lifetime fraction (D) in Mn²⁺ of ICAM-1 bonds with initially extended or bent α_Lβ₂ before loading, which underwent a bending or unbending conformational change after loading before dissociation. (E and F) Postbending and postunbending (t₁) short (<10 s) lifetime mean (E) and long (>10 s) lifetime fraction (F) of α_Lβ₂–ICAM-1 bonds versus postswitch force (f₁) in Mn²⁺. Fractions in B, D, and F refer to the percentages of events with a lifetime >10 s in all lifetime measurements. (G) Summary of α_Lβ₂ conformations and conformational changes in different groups, which are also depicted along side of B, D, and F with legends to help data interpretation. Error bars represent SEM of >50 measurements.