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. 1980 Jan;77(1):563–566. doi: 10.1073/pnas.77.1.563

Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.

R S Accolla, S Carrel, J P Mach
PMCID: PMC348313  PMID: 6153797

Abstract

Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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