Table 1.
KL1 genome annotation
| Gene | Start | End | Putative function | Strand | Predicted ribosome binding site and start codon | Length (amino acids) | Closest relative | Alignment region (amino acids) | Percent identity | Source | GenBank accession number |
|---|---|---|---|---|---|---|---|---|---|---|---|
|
1 |
1 |
267 |
unknown |
+ |
AGGGGCGAActtcgtATG |
88 |
hypothetical protein ORF001 |
1-84/84 |
77 |
Pseudomonas phage 73 |
YP_001293408.1 |
|
2 |
264 |
560 |
holin |
+ |
AAAGGGGCGGtaacGTG |
98 |
hypothetical protein ORF002 |
3-88/88 |
42 |
Pseudomonas phage 73 |
YP_001293409.1 |
|
3 |
514 |
1080 |
lysin |
+ |
AAAAGGGGttatcgaATG |
188 |
hypothetical protein bglu_1g27070 |
2-181/188 |
47 |
Burkholderia glumae BGR1 |
YP_002912484.1 |
|
4 |
1091 |
1408 |
Rz |
+ |
AAGTAAGGGGttcgaaATG |
105 |
hypothetical protein ORF004 |
1-101/101 |
37 |
Pseudomonas phage 73 |
YP_001293411.1 |
|
5 |
1329 |
1592 |
Rz1 |
+ |
GAAAGGtgccgccgATG |
87 |
conserved hypothetical protein |
1-79/86 |
40 |
Burkholderia sp. Ch1-1 |
ZP_06842908.1 |
|
6 |
1647 |
2138 |
unknown |
+ |
ACTAGGccgcgattATG |
163 |
hypothetical protein ORF005 |
1-162/162 |
59 |
Pseudomonas phage 73 |
YP_001293412.1 |
|
7 |
2116 |
3756 |
terminase large subunit |
+ |
AACAGGAAttgcttaATG |
546 |
hypothetical protein ORF006 |
10-531/531 |
84 |
Pseudomonas phage 73 |
YP_001293413.1 |
|
8 |
3770 |
5266 |
portal protein |
+ |
AAAGGAAAcgaaatcATG |
498 |
hypothetical protein ORF007 |
3-494/501 |
85 |
Pseudomonas phage 73 |
YP_001293414.1 |
|
9 |
5269 |
6384 |
head morphogenesis protein |
+ |
GGGGCGTAatcATG |
371 |
hypothetical protein ORF008 |
1-364/364 |
73 |
Pseudomonas phage 73 |
YP_001293415.1 |
|
10 |
6403 |
7110 |
unknown |
+ |
AAGGAGtccttgaaATG |
235 |
hypothetical protein ORF009 |
1-235/239 |
82 |
Pseudomonas phage 73 |
YP_001293416.1 |
|
11 |
7123 |
8097 |
major capsid protein |
+ |
AAGGAcactttatcATG |
324 |
hypothetical protein ORF010 |
1-325/325 |
90 |
Pseudomonas phage 73 |
YP_001293417.1 |
|
12 |
8171 |
8587 |
unknown |
+ |
AAGGAGtttcgaacATG |
138 |
hypothetical protein ORF011 |
1-134/134 |
69 |
Pseudomonas phage 73 |
YP_001293418.1 |
|
13 |
8656 |
9033 |
unknown |
+ |
AAAGGAGcgtcgaacATG |
125 |
hypothetical protein ORF012 |
1-123/123 |
70 |
Pseudomonas phage 73 |
YP_001293419.1 |
|
14 |
9047 |
9565 |
unknown |
+ |
AAGGGGcgcggcatcATG |
172 |
hypothetical protein ORF013 |
1-172/172 |
83 |
Pseudomonas phage 73 |
YP_001293420.1 |
|
15 |
9570 |
9944 |
head-tail joining protein |
+ |
GATAAGGGtctaacgctATG |
124 |
hypothetical protein ORF014 |
1-124/126 |
59 |
Pseudomonas phage 73 |
YP_001293421.1 |
|
16 |
9941 |
10399 |
minor tail protein |
+ |
ATACGGTAttgttcgcacaATG |
152 |
hypothetical protein ORF015 |
5-151/151 |
68 |
Pseudomonas phage 73 |
YP_001293422.1 |
|
17 |
10412 |
11965 |
unknown |
+ |
AAGGAGttacgaaaATG |
517 |
hypothetical protein ORF016 |
3-511/511 |
78 |
Pseudomonas phage 73 |
YP_001293423.1 |
|
18 |
12030 |
12458 |
tail protein |
+ |
GGAGTAAAccaaATG |
142 |
hypothetical protein ORF017 |
1-142/142 |
79 |
Pseudomonas phage 73 |
YP_001293424.1 |
|
19 |
12030 |
12823 |
tail protein |
+ |
GGAGTAAAccaaATG |
264 |
hypothetical protein ORF017 |
1-142/142 |
79 |
Pseudomonas phage 73 |
YP_001293424.1 |
| |
|
|
|
|
|
|
hypothetical protein ORF018 |
1-118/118 |
78 |
Pseudomonas phage 73 |
YP_001293425.1 |
|
20 |
12792 |
13226 |
tail protein |
+ |
AAAAGGCGGcgcaacagaATG |
144 |
hypothetical protein ORF019 |
1-144/144 |
80 |
Pseudomonas phage 73 |
YP_001293426.1 |
|
21 |
13232 |
17050 |
tail tape measure |
+ |
AAGGAttagcagaaATG |
1272 |
hypothetical protein ORF020 |
1-78, 131-1202/1204 |
61, 57 |
Pseudomonas phage 73 |
YP_001293427.1 |
|
22 |
17069 |
18067 |
unknown |
+ |
AGGAAtacgaattATG |
332 |
hypothetical protein XALc_0225 |
1-295/307 |
30 |
Xanthomonas albilineans GPE PC73 |
YP_003374757.1 |
|
23 |
18070 |
19179 |
unknown |
+ |
GAGGAAAActaatcATG |
369 |
hypothetical protein ORF033 |
1-332/333 |
25 |
Pseudomonas phage M6 |
YP_001294541.1 |
|
24 |
19179 |
20870 |
tail assembly protein |
+ |
AAGAAGAtcgcataATG |
563 |
hypothetical protein ORF023 |
63-565/568 |
36 |
Pseudomonas phage 73 |
YP_001293430.1 |
|
25 |
20867 |
21688 |
tail assembly protein |
+ |
AAGGAcgattccagaATG |
273 |
hypothetical protein ORF024 |
1-273/274 |
49 |
Pseudomonas phage 73 |
YP_001293431.1 |
|
26 |
21689 |
24100 |
tail assembly protein |
+ |
AAGATGGGGtcggttaaATG |
803 |
hypothetical protein ORF025 |
1-755/813 |
49 |
Pseudomonas phage 73 |
YP_001293432.1 |
|
27 |
24097 |
26166 |
DNA polymerase |
- |
AAGGAAtttgcccgATG |
689 |
hypothetical protein ORF026 |
1-682/683 |
83 |
Pseudomonas phage 73 |
YP_001293433.1 |
|
28 |
26179 |
27339 |
DNA polymerase III β subunit |
- |
AAGGGGttaaaaATG |
386 |
hypothetical protein ORF027 |
2-380/380 |
74 |
Pseudomonas phage 73 |
YP_001293434.1 |
|
29 |
27323 |
27691 |
unknown |
- |
GAATGGtgaaattATG |
122 |
hypothetical protein Dole_2913 |
5-84/87 |
33 |
Desulfococcus oleovorans Hxd3 |
YP_001530793.1 |
|
30 |
27696 |
29351 |
superfamily II helicase/restriction enzyme |
- |
AAGGGttacgaATG |
551 |
hypothetical protein ORF029 |
1-551/551 |
90 |
Pseudomonas phage 73 |
YP_001293436.1 |
|
31 |
29344 |
30342 |
exonuclease |
- |
GGAAGGcgaagaacgATG |
332 |
hypothetical protein ORF030 |
1-365/365 |
65 |
Pseudomonas phage 73 |
YP_001293437.1 |
|
32 |
30852 |
31637 |
unknown |
- |
GAAAGGtgaaacgaacATG |
261 |
hypothetical protein Isop_2441 |
1-118/151 |
37 |
Isosphaera pallida ATCC 43644 |
YP_004179564.1 |
|
33 |
31696 |
32412 |
recombinase |
- |
AGGTGAAcgtATG |
238 |
hypothetical protein ORF032 |
1-238/238 |
91 |
Pseudomonas phage 73 |
YP_001293439.1 |
|
34 |
32471 |
32980 |
unknown |
- |
AAGGAAccccaaaATG |
169 |
hypothetical protein ORF033 |
7-146/146 |
49 |
Pseudomonas phage 73 |
YP_001293440.1 |
|
35 |
33059 |
33598 |
pyrophosphohydrolase |
- |
AGGGGcatcgtATG |
179 |
hypothetical protein ORF034 |
8-185/185 |
69 |
Pseudomonas phage 73 |
YP_001293441.1 |
|
36 |
33746 |
33934 |
transcriptional regulator |
+ |
GGGGcaagcATG |
62 |
hypothetical protein ORF035 |
1-61/62 |
51 |
Pseudomonas phage 73 |
YP_001293442.1 |
|
37 |
33924 |
36233 |
primase |
+ |
GAAGGcttgcgcaaatATG |
769 |
hypothetical protein ORF036 |
1-773/773 |
85 |
Pseudomonas phage 73 |
YP_001293443.1 |
|
38 |
36366 |
36668 |
unknown |
+ |
GAAGGAgttacgaacATG |
100 |
hypothetical protein |
132-217/217 |
44 |
Deftia phage ϕW-14 |
YP_003359005.1 |
|
39 |
36735 |
37091 |
unknown |
+ |
GAAGGAGtacacgccATG |
118 |
unnamed protein product |
262-336/404 |
32 |
Azospirillum lipoferum 4B |
YP_004974060.1 |
|
40 |
37097 |
37360 |
unknown |
+ |
AGAAGAAGGAGtaagcgccATG |
87 |
PREDICTED: photosystem II reaction center PSB28 protein, chloroplastic |
22-86/179 |
32 |
Vitis vinifera |
XP_002271666.1 |
|
41 |
37728 |
38024 |
unknown |
+ |
AAAGGAGcgccagccATG |
98 |
hypothetical protein ORF039 |
1-97/98 |
70 |
Pseudomonas phage 73 |
YP_001293446.1 |
|
42 |
38060 |
38296 |
unknown |
+ |
AAGGAAccccgatcATG |
78 |
hypothetical protein ORF040 |
1-80/80 |
50 |
Pseudomonas phage 73 |
YP_001293447.1 |
|
43 |
38302 |
38703 |
unknown |
+ |
AAAGGGGtaattactATG |
133 |
hypothetical protein ORF042 |
1-120/124 |
40 |
Pseudomonas phage 73 |
YP_001293449.1 |
|
44 |
38707 |
39195 |
Vsr endonuclease |
+ |
GACGAAGttgcattaagccATG |
162 |
hypothetical protein ORF043 |
1-176/179 |
61 |
Pseudomonas phage 73 |
YP_001293450.1 |
|
45 |
39201 |
39458 |
unknown |
+ |
GGAAGGAGtaacccaaATG |
85 |
hypothetical protein Astex_0306 |
3-81/183 |
44 |
Asticcacaulis excentricus CB 48 |
YP_004086155.1 |
|
46 |
39455 |
39655 |
unknown |
+ |
GGCGAAGtcgtcgaATG |
66 |
monooxygenase, FAD-binding |
385-445/546 |
38 |
Streptomyces griseoflavus Tu4000 |
ZP_07309792.1 |
|
47 |
39652 |
39840 |
unknown |
+ |
AAGGAGtacgcaccATG |
62 |
hypothetical protein METUNv1_00516 |
11-65/68 |
39 |
Methyloversatilis universalis FAM5 |
ZP_08503515.1 |
|
48 |
39882 |
40154 |
unknown |
+ |
AAAAGGAGtaacgaacATG |
90 |
hypothetical protein Cflav_PD2164 |
58-133/172 |
30 |
bacterium Ellin514 |
ZP_03630603.1 |
|
49 |
40138 |
40374 |
unknown |
+ |
GAACCGGAttacgattATG |
78 |
hypothetical protein ORF047 |
2-77/77 |
67 |
Pseudomonas phage 73 |
YP_001293454.1 |
|
50 |
40374 |
40550 |
unknown |
+ |
GGGTTAcgaataATG |
58 |
hypothetical protein Glaag_3667 |
90-140/227 |
29 |
Glaciecola sp. 4 H-3-7 + YE-5 |
YP_004435864.1 |
|
51 |
40562 |
40933 |
unknown |
+ |
GAAAGGtgaaatcATG |
123 |
hypothetical protein BURMUCGD2M_4586 |
8-67/70 |
34 |
Burkholderia multivorans CGD2M |
ZP_03569237.1 |
|
52 |
40930 |
41415 |
dCMP deaminase |
+ |
GGAACGtccggcATG |
161 |
hypothetical protein ORF049 |
2-153/155 |
75 |
Pseudomonas phage 73 |
YP_001293456.1 |
|
53 |
41412 |
41786 |
unknown |
+ |
AAAGGctgaatcATG |
124 |
hypothetical protein ORF050 |
4-125/127 |
43 |
Pseudomonas phage 73 |
YP_001293457.1 |
|
54 |
41826 |
42032 |
unknown |
+ |
GGGGAtgcccacattATG |
68 |
hypothetical protein ORF051 |
37-94/94 |
45 |
Pseudomonas phage 73 |
YP_001293458.1 |
| 55 | 42120 | 42674 | unknown | + | AAGGAGttttacaaATG | 184 | hypothetical protein ORF052 | 9-190/190 | 66 | Pseudomonas phage 73 | YP_001293459.1 |