Table 2.
Polymorphic SSRs primer pairs derived from N. nervosa unigenes
| ID name | Locus | Repeat motif | ORF | Forward and Reverse Primers | Amplicon length observed | BLASTX, seq description | Seq Lenght (bp) | Sim mean (%) | GO terms related to response to stress |
|---|---|---|---|---|---|---|---|---|---|
| isotig00192 |
INTANOT1 |
(tct)5 |
Y |
F: CCAGATGGGTTTTTGCTTGT |
148 |
heat shock protein 81-1 |
2309 |
97.2 |
response to stimulus |
| R: GACGATGAAGACGATGAGC | |||||||||
| isotig00230 |
INTANOT2 |
(tcg)5 |
N |
F: TTTCCAAACGGTTCCAGAAG |
120 |
af367280_1at3g56860 t8m16_190 |
1229 |
76.6 |
response to stress |
| R:AACGGAGAAGGATGTTTCCA | |||||||||
| isotig00551 |
INTANOT3 |
(tcattt)3 |
Y |
F: CCGATGTGATCGATAGGCTT |
204 |
ac005850_9highly simlilar to mlo proteins |
1759 |
77.5 |
defense response to fungus |
| R: CATGTCCCCAGTTCACCTCT | |||||||||
| isotig00597 |
INTANOT4 |
(ta)6 |
N |
F:AAAACACCACCAAACCCAAA |
197 |
dnaj heat shock n-terminal domain-containing protein |
1516 |
78.3 |
response to stimulus |
| R: CTTTGCCACGGCAACTAAAT | |||||||||
| isotig01207 |
INTANOT5 |
(tct)7 |
N |
F: CTCGAAGACGCTACCAGACC |
280 |
af214107_1 -like protein |
748 |
79.3 |
response to stimulus |
| R: TCCTGGGTTTTGCATATTGG | |||||||||
| isotig01232 |
INTANOT6 |
(atc)4 |
Y |
F: CGTTTCCCTTTAGCTGATGC |
173 |
aldh6b2 3-chloroallyl aldehyde dehydrogenase methylmalonate-semialdehyde dehydrogenase oxidoreductase |
741 |
96.8 |
response to stress |
| R:GCTGAGTTAGCAATGGAGGC | |||||||||
| GR7D2IN01BK031 |
INTANOT7 |
(ag)5 |
N |
F: GACGACATCGTTCCGAGTTT |
241 |
f-box family protein |
536 |
75.4 |
response to heat |
| R: GTTAATCCCTCTCTCCTCAT | |||||||||
| GR7D2IN01CGQUT |
INTANOT8 |
(ccgaaa)3 |
Y |
F: CTCCCTCAAACACCTCCAAA |
236 |
mitogen-activated protein kinase kinase |
518 |
90.5 |
response to osmotic stress |
| R: ATTCAAGTGGGTCTTGCCTG | |||||||||
| GR7D2IN01EMGE0 |
INTANOT9 |
(ct)8 |
N |
F: CCGGCTACCTGTTTGTTTTA |
155 |
at1g78870 f9k20_8 |
507 |
100.0 |
response to metal ion |
| R: TTCCTTGATGATTCTTCGGG | |||||||||
| GR7D2IN02FPPC7 |
INTANOT10 |
(ggt)6 |
Y |
F: AAAATTGCTGTTGAGGGTGG |
117 |
af361609_1at1g27760 t22c5_5 |
529 |
87.9 |
response to osmotic stress |
| R: CCTGAATCACCAGACCGAC | |||||||||
| GR7D2IN02GFAUT |
INTANOT11 |
(gaa)4 |
Y |
F: ATCCCCAATCTTTCCCAATC |
115 |
salt overly sensitive 1 |
315 |
78.5 |
response to reactive oxygen species; response to osmotic stress |
| R: AATTCTGTCCGCTTTGGCTA | |||||||||
| GR7D2IN02GR6NZ |
INTANOT12 |
(at)5 |
Y |
F: TCTTGTGGCAAGTGCTTGAG |
285 |
win2_soltu ame: full = wound-induced protein win2 flags: precursor |
472 |
94.0 |
defense response |
| R: ACTATCCTCACCGTTGCCTG | |||||||||
| GR7D2IN02HOKOI |
INTANOT13 |
(tc)5 |
Y |
F: ATATCCTGGAAATGCTTGCG |
124 |
exec1_arath ame: full = protein executer chloroplastic flags: precursor |
469 |
71.7 |
response to reactive oxygen species |
| R: TAAACGATCTTCGGAATGGC | |||||||||
| GR7D2IN02HWXOR |
INTANOT14 |
(tgg)8 |
Y |
F: AGGAGCTAAATGGGCGTAA |
260 |
glycine-rich rna-binding protein |
452 |
86.5 |
response to stress |
| R: CACCACCACCACCAAAGAA |
Included are ID names, primer names, motive and number of repeats, position in ORF, sequence of forward and reverse primers (5′3′), amplicon length (bp), BLASTX similarity matches (Putative Function), Sequence length, Similarity Mean (%), GO terms related to stress response.