Table II.
Mass enrichment of proposed intermediates of SA biosynthesis from [13C1]phenylalanine
| Time | Pool | CA | BD | BA | SA |
|---|---|---|---|---|---|
| h | % | ||||
| 2 | F | 74 ± 1 | n.d.a | 17 ± 7 | 58 ± 9 |
| T | 72 ± 2 | 34 ± 7 | 55 ± 12 | ||
| 4 | F | 73 ± 3 | n.d. | 12 ± 4 | 57 ± 4 |
| T | 71 ± 9 | 29 ± 7 | 58 ± 7 | ||
| 24 | F | 47 ± 13 | n.d. | 3 ± 1 | 17 ± 4 |
| T | 70 ± 5 | 29 ± 4 | 52 ± 10 | ||
Leaf discs from TMV-inoculated plants held at 32°C for 96 h were infiltrated with a 1 mm solution of 3-[13C1]phenylalanine 6 h after a temperature shift to 24°C. BD was analyzed for enrichment at 2, 4, and 24 h after infiltration, as well as for the free (F) and total (T) pools of CA, BA, and SA. The enrichment value is the percentage of 13C-labeled compound from its total pool ± se.
a
n.d., Signifies nondetectable amounts of enrichment.