Figure 1. Calcitriol modulates CD46 expression in CD4+ T cells.
Purified CD4+ T cells from healthy donors were left unstimulated (US), or were stimulated by immobilized anti-CD3, anti-CD3/CD28, or anti-CD3/CD46 antibodies as indicated in presence of calcitriol (10−7M) or ethanol as vehicle control. CD46 expression was monitored by flow cytometry. The representative expression of CD46 after 5 days of culture is shown in (A). The average expression of CD46 detected after 2 or 5 days for the different donors analyzed (mean ± SEM; n = 15) is shown in (B). Samples were analyzed using the Wilcoxon test.