Table 5.

Foxp3 pyrosequencing/primer design

	Foxp3 promoter	Foxp3 intronic region
Forward	5′-CAAATTCAGAGTATTAGTTCTTTTCCTCTTT−3′	5′-TTGGGTTAAGTTTGTTGTAGGAT−3′
Reverse	5′-[Biotin]-GTGGCATGTCGCACCAAAAAGAAGAG−3′	5′-[Biotin]- ACCCCCCACTTACCCAAATTTT−3′
Sequencing	5′-AAAGGCAAATTCA-3′	5′-GTAGGATAGGGTAGTTAG-3′
Description	6 CpG sites*	7 CpG sites*
	(-141, -95, -80, -66, -54, -27)	(+3951, +3956, +4105, +4224, +4228, +4236, +4245)

*CpG sites indicate nucleotide position in relation to transcription start. Symbols – and + indicate upstream and downstream of transcription start, respectively. CpG, cytosine nucleotide neighboring guanine.