Figure 3. dsbA knockout mutant and ToxR forms.

Immunoblot analyses are shown using anti-FLAG antibodies to detect FLAG-tagged ToxR produced in V. cholerae P27459-S ΔtoxRS and ΔtoxRS ΔdsbA mutant strain (as indicated in the figure). Bacterial cultures harboring pFLAGtoxRS were grown to mid-log phase in M9 glycerol and in LB broth and induced with IPTG. ToxR mobility in the different samples was monitored and differences for intrachain disulfide bond formation were detected. Immunoblot analysis was performed at least three times, and results were reproducible.