|
E. coli
strains
|
|
|
| XL1-Blue |
F′::Tn10 proA
+
B
+
lacq Δ(lacZ)M151 recA1 endA1 gyrA46 (Nalr) thi hsdR17 (rK
−mK
+) supE44 relA1 lac
|
NEB |
| DH5αλpir |
F− Φ80dlacZΔM15Δ(argF lac)U169 deoR recA1 endA1 hsdR17 (rK
−mK
+) supE44 thi-1 gyrA69 relA1 λ recA::RPA-2-Te::Mu λpirR6K, kmr
|
[69]
|
| SM10λpir |
thi thr leu tonA lacY supE recA::RPA-2-Te::Mu λpirR6K, kmr
|
[25]
|
|
V. cholerae
strains
|
|
|
| O395 |
O1 Ogawa, classical, clinical isolate, India 1964, spontaneous smr
|
[41]
|
|
P27459-S |
O1 Inaba, El Tor, clinical isolate, Bangladesh 1976, spontaneous smr
|
[40]
|
| O395 ΔdsbA::km |
dsbA replaced by km cassette, smr, kmr
|
This study |
| O395 ΔdsbB::km |
dsbB replaced by km cassette, smr, kmr
|
This study |
| O395 ΔtoxR
|
Deletion in toxR, smr
|
This study |
| O395 ΔtoxRS
|
Deletion in toxR and toxS, smr
|
This study |
|
P27459-S ΔdsbA::km |
dsbA replaced by km cassette, smr, kmr
|
This study |
|
P27459-S ΔdsbA::km ΔtoxRS
|
dsbA replaced by km cassette, deletion of toxR and toxS, smr, kmr
|
This study |
|
P27459-S ΔdsbB::km |
dsbB replaced by km cassette, smr, kmr
|
This study |
|
P27459-S dsbC::pGP |
dsbC inserted by pGP704, amr
|
This study |
|
P27459-S ΔtoxR
|
Deletion in toxR, smr
|
This study |
|
P27459-S ΔtoxRS
|
Deletion in toxR and toxS, smr
|
This study |
| O395 ΔtoxR::FLAGtoxR
|
toxR replaced by FLAGtoxR, smr
|
This study |
| O395 ΔtoxR::FLAGtoxRCC
|
toxR replaced by FLAGtoxRC236SC293S, smr
|
This study |
| O395 ΔtoxR::FLAGtoxR ΔtoxS
|
toxR replaced by FLAGtoxR, deletion in toxS, smr
|
This study |
|
P27459-S ΔtoxR::FLAGtoxR
|
toxR replaced by FLAGtoxR, smr
|
This study |
|
P27459-S ΔtoxR::FLAGtoxRCC
|
toxR replaced by FLAGtoxRC236SC293S, smr
|
This study |
|
P27459-S ΔtoxR::FLAGtoxR ΔlacZ
|
toxR replaced by FLAGtoxR, deletion in lacZ, smr
|
This study |
|
P27459-S ΔtoxR::FLAGtoxR ΔtoxS
|
toxR replaced by FLAGtoxR, deletion in toxS, smr
|
This study |
|
Plasmids
|
|
|
| pKEK229 |
OriR6K
mobRP4 sacB, apr
|
[70]
|
| pGP704 |
OriR6K
mobRP4, apr
|
[25]
|
| pCVD442 |
OriR6K
mobRP4 sacB, apr
|
[45]
|
| pKan π |
kmr
|
[71]
|
| pBAD18 |
Expression vector, arabinose inducible, apr
|
[72]
|
| pFLAG-MAC™ |
Expression vector with N-terminal FLAG-Tag, IPTG inducible, apr
|
Sigma-Aldrich |
| pGPdsbC |
pGP704 carrying internat fragment of dsbC′ apr
|
This study |
| pKEK229dsbA::km |
pCVD442 carrying up and down fragment of dsbA flanking a km cassette, apr
|
This study |
| pKEK229dsbB::km |
pCVD442 carrying up and down fragment of dsbB flanking a km cassette, apr
|
This study |
| pCVD442lacZ |
pCVD442 carrying up and down fragments of lacZ, apr
|
This study |
| pCVD442toxR |
pCVD442 carrying up and down fragment of toxR, apr
|
This study |
| pCVD442toxRS |
pCVD442 carrying up fragment of toxR and down fragments of toxS, apr
|
This study |
| pdsbA |
dsbA of P27459-S in pBAD18, apr
|
This study |
| pdsbB |
dsbB of P27459-S in pBAD18, apr
|
This study |
| pFLAGtoxR |
toxR of P27459-S in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRCC
|
toxRC236SC293S point mutant of P27459-S in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRS |
toxR and toxS of P27459-P in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRCCS |
toxRC236SC293S point mutant and toxS of P27459-P in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRS(Δ264) |
pFLAGtoxRS carrying a 264 bp deletion in toxS generated by two internal AccI sites, apr
|
This study |
| pFLAGtoxRS_ompU |
toxR, toxS and operator region of ompU of P27459-P in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRS_toxT |
toxR, toxS and operator region of toxT of P27459-P in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRS_ompU(Δ264) |
pFLAGtoxRS_ompU carrying a 264 bp deletion in toxS generated by two internal AccI sites, apr
|
This study |
| pFLAGtoxRS_toxT(Δ264) |
pFLAGtoxRS_toxT carrying a 264 bp deletion in toxS generated by two internal AccI sites, apr
|
This study |
| pFLAGtoxRCCS_ompU |
toxRC236SC293S point mutant, toxS and operator region of ompU of P27459-P in pFLAG-MAC™, apr
|
This study |
| pFLAGtoxRCCS_toxT |
toxRC236SC293S point mutant, toxS and operator region of toxT of P27459-P in pFLAG-MAC™, apr
|
|
| pCVD442FLAGtoxR |
pCVD442 carrying FLAGtoxR, apr
|
|
| pCVD442FLAGtoxRCC
|
pCVD442 carrying FLAGtoxRC236SC293S, apr
|
|
| pCVD442FLAGtoxRS |
pCVD442 carrying FLAGtoxR and toxS, apr
|
|
