Table 2. Oligonucleotide primers.
| Oligonucleotides | Sequence (5′ - 3′)a |
| SacI_dsbA_1 | TTTGAGCTCCAAGAAGAGATCCCGATCGTCC |
| EcoRI_dsbA_2 | TTTGAATTCCATGACTTTCTCCATTGGATTTATT |
| EcoRI_dsbA_3 | AATGAATTCTAATCTCAACCCATGATTCGGTAT |
| XbaI_dsbA_4 | TTTTCTAGAGATTAAACTGTTGCTGCCGTCAG |
| SacI_dsbB_1 | TTTGAGCTCGTCTTCCTGCCAATGTT |
| EcoRI_dsbB_2 | TTTGAATTCCACAGATAGATCCTTGTTAAAAAGA |
| EcoRI_dsbB_3 | TTTGAATTCTAAGCCAATCGCATCGCTCAAT |
| XbaI_dsabB_4 | TTTTCTAGATAGCATGGAGAGTGAGCCGCCACT |
| EcoRI_dsbC_1 | ATTGAATTCGTGCAAACGTCTGGTGGT |
| XbaI_dsbC_2 | ATTTCTAGAGAGCTCGTGACCCAGCAT |
| SacI_toxRS_1 | TTTGAGCTCATTTGGAAATCACATCGCGCAAAC |
| BamHI_toxRS_2 | TTTGGATCCTCCTAATCCGAACATCTAATGTCC |
| BamHI_toxR_3 | TTTGGATCCAACCCTAACGATGCCATCAAAGT |
| BamHI_toxRS_3 | TTTGGATCCTCCGATGACAATAGTGCAGAAAG |
| XbaI_toxRS_4 | TTTTCTAGAATGACGTTTCCCCGCGGTGAG |
| XbaI_lacZ_1 | TAATCTAGAACACATAACCCTGCAGTA |
| XhoI_lacZ_2 | TTTCTCGAGCTCTACGGCGTACATCCCT |
| XhoI_lacZ_3 | TTTCTCGAGTGCGTGTGGAATGTGACGAT |
| SacI_lacZ_4 | ATGAGCTCTTATTGTGGGGATGACGCTTT |
| SacI_dsbA_5′ | AATGAGCTCGCCACTTTACAAGAACCCCCG |
| XbaI_dsbA_3′ | ATTTCTAGAGATTTACAAAGCCGATTAGCACTG |
| SacI_dsbB_5′ | AATGAGCTCCAATTGAAACTGAAACTAATCCAAG |
| XbaI_dsbB_3′ | AATTCTAGACTTTAAGCGCCTTTTTTATCAACC |
| HindIII_toxR_5′_FLAG | AATAAGCTTATGTTCGGATTAGGACACAACTCA |
| KpnI_toxR_3′_FLAG | AATGGTACCCTACTCACACACTTTGATGGCAT |
| KpnI_toxRC293S_3′_FLAG | AATGGTACCCTACTCAGACACTTTGATGGCATCGTTA b |
| toxRC236S_5′ | GGCTACCGTCAATCGAACTGAGCGTTAAAAAATACAATGA b |
| toxRC236S_3′ | TCATTGTATTTTTTAACGCTCAGTTCGATTGACGGTAGCC b |
| KpnI_toxRS_5′_FLAG | AATGGTACCCATGTTCGGATTAGGACACAACTCA |
| BglII_toxRS_3′_FLAG | TTAAGATCTTTAAGAATTACTGAACAGTACGGT |
| BamHI_ompU_5′ | ATTGGATCCTCCTAAATCGGGTCGGGTT |
| BamHI_ompU_3′ | AATGGATCCGGCTCAGCCATTTTCGTGGC |
| BamHI_toxT_5′ | TTAGGATCCGTATAGCAAAGCATATTCAGAGA |
| BamHI_toxT_3′ | ATTGTCGACTAAATAAACGCAGAGAGCCATC |
| c_FLAGtoxR_3′_F1 | TGTCATCGTCGTCCTTGTAGTCCATCTAATGTCCCAGTATCTCCCTGT |
| c_FLAGtoxR_5′_F2 | GGGACAGGGAGATACTGGGACATTAGATGGACTACAAGGACGACGATGA |
| c_FLAGtoxR_3′_F2 | CTACTCACACACTTTGATGGCAT |
| c_FLAGtoxRC293S_3′_F2 | CTACTCAGACACTTTGATGGCAT b |
| c_FLAGtoxRtoxS_3′_F2 | CTTTCTGCACTATTGTCATCGGTCTACTCACACACTTTGATGGCAT |
| c_FLAGtoxR_5′_F3 | AACCAGTTAACGCTGAATTACATTC |
| c_FLAGtoxRC293S_5′_F3 | GTTGCTAACCCTAACGATGCCATCAAAGTGTCTGAG b |
| c_FLAGtoxRtoxS_5′_F3 | ATGCCATCAAAGTGTGTGAGTAGTCCGATGACAATAG |
| rpoB_fwc | CTGTCTCAAGCCGGTTACAA |
| rpoB_rvc | TTTCTACCAGTGCAGAGATGC |
| VC0633_fw | CTCGCGTACGTCTAAACTTCTTGG |
| VC0633_rv | CGGTTGTCTAGGCTGTTGTTAGAC |
| VC0984_fw | CCTTCATCAGCCACTGTAGTGAAC |
| VC0984_rv | GACCGCTATCAGAATAAGCAGTCG |
| VC1854_fw | ACCCACTAGTGATCGATGAAGACG |
| VC1854_rv | GCCATACTCAGCATATACACGAGC |
| VCr001_fw | AGGGAGGAAGGTGGTTAAGT |
| VCr001_rv | CGCTACACCTGAAATTCTACCC |
a
Restriction sites are underlined.
b
Bold letters indicate codons changed to obtain desired amino acid mutations.
c
Oligonucleotides for rpoB are according to reference [73].