Table 3. Virulence factor production of chromosomal encoded FLAG-tagged toxR and FLAG-tagged toxR^CC mutants.

Strain	CTX-kmΦ transduction rate [cfu×ml−1]a	CT production [ng×ml−1]b
El Tor P27459-S
ΔtoxR::FLAGtoxR	4.76×10−5 (1.5×10−5–1.03×10−4)	7502 (4341–15734)
ΔtoxR	<LOD	92 * (77–127)
ΔtoxR::FLAGtoxRCC	3.83×10−5 (2.48×10−5–6.84×10−5)	5012 (2433–11578)
ΔtoxR::FLAGtoxR ΔtoxS	2.96×10−6 (8.15×10−7–2.76×10−5)	1543 * (988–2570)
WT	4.54×10−5 (2.85×10−5–6.09×10−5)	4654 (2605–7806)
Classical O395
ΔtoxR::FLAGtoxR	1.55×10−4 (2.78×10−5–1.98×10−4)	17178.5 (10372–40044)
ΔtoxR	<LOD	10 * (10–10)
ΔtoxR::FLAGtoxRCC	5.71×10−5 (1.63×10−5–3.13×10−4)	12725.5 (3961–48650)
ΔtoxR::FLAGtoxR ΔtoxS	2.22×10−5 (8.87×10−6–1.01×10−4)	5490 (3042–15710)
WT	1.34×10−4 (2.02×10−5–5.60×10−4)	11354.5 (9978–25108)

^amedian and interquartile range of at least 7 independent experiments.

^bmedian and interquartile range of 9 independent experiments.

^*significant by Kruskal-Wallis test followed by Dunn's test of selected pairs of columns with P<0.05.

<LOD below limit of detection of 5×10⁻⁸.