Table 1.
Detection of Flavivirus genome in BAGV infected game birds
| Sample |
Partridge |
Pheasant |
Wood Pigeon |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 1 | 2 | 3 | 4 | 5 | 1 | 2 | |
| Oral swab |
19 |
− |
30 |
23* |
− |
19* |
− |
− |
29 |
31 |
− |
− |
− |
| Clocacal swab |
− |
− |
− |
− |
− |
29 |
− |
− |
− |
31 |
− |
− |
− |
| Heart |
− |
− |
32 |
23 |
− |
23 |
− |
− |
− |
− |
27 |
31 |
− |
| Kidney |
29 |
30 |
− |
− |
− |
− |
28 |
− |
29 |
27 |
29 |
29 |
30 |
| Brain |
− |
22* |
22* |
16*† |
30 |
− |
26* |
23* |
24* |
30 |
30 |
30 |
− |
| Spleen | 30 | − | − | − | − | 30 | − | − | − | − | − | 27 | − |
*Sequenced 104 bp amplicon from conventional PCR.
† Sequenced 1048 bp amplicon from conventional PCR.
− Samples that did not show amplification.
Detection of flavivirus genome by rtRT-PCR (Ct values) and by sequencing from conventional PCR in different tissues and swabs.