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The thermal shift assay of NanA in seven different buffers. The melting curves of NanA undergo unfolding with the increasing of temperature in the tested buffers (Red Acetate, Orange MOPS, Blue Phosphate, Green MES, Cyan HEPES, Black Tris-Cl, and Purple Imidazole). MES buffer (Green line) confers the optimal sialidase thermal stability for NanA.
