Table 3.
Primer sequences used for RT-qPCR
| Gene symbol | Entrez Gene ID | Forward primer 5' -› 3' | Reverse primer 5' -› 3' | ATa |
|---|---|---|---|---|
| mRNA quantification | ||||
|
fabp1a |
791610 |
TAAGCTGACAGCGTTTGTGAAGGG |
AGATGCGTCTGCTGATCCTCTTGT |
60.0 |
|
fabp1b.1 |
554095 |
AAGCTGAAGGTGGTGCTGAACA |
CACGTTTGCTGATGCGCTTGTA |
59.0 |
|
fabp1b.2 |
EB880179 |
TGCCGTTCTCTGGGAAGTTTGAGT |
TGACTTTGTCTCCGCTCAGCATCT |
61.0 |
|
fabp7a |
58128 |
TGTGCCACTTGGAAACTGGTTGAC |
AACATTGCCTACTTGCCTGGTAGG |
60.0 |
|
fabp7b |
407736 |
AAACCACTGCTGATGACCGACACT |
AGTGGTCTCTTTCCCATCCCACTT |
61.0 |
|
fabp10a |
171481 |
TTACGCTCAGGAGAACTACG |
CTTCCTGATCATGGTGGTTC |
55.0 |
|
fabp10b |
795210 |
CGGCTCCAGAGCACTACATC |
GTTCACTCATGTGCGGGAGC |
60.0 |
|
fabp11a |
447944 |
TGTGCAGAAACAGACCTGGGA |
ACAGCCACCACATCACCCATCTT |
60.0 |
|
fabp11b |
553579 |
GCTGTCACTACATTCAAGACCTG |
AGTTTACCATCCGCAAGGCTCA |
60.0 |
|
acox1 |
449662 |
AGTCAGCACGAGCTCTCTCC |
GCCCTACAAAGTGAAAGGCA |
58.0 |
|
rpl13α |
560828 |
AGCAAGTGCTGTTGGGCCAC |
GTGTGGCGGTGATGGCCTGG |
61.0 |
| hnRNA quantification | ||||
|
fabp1a |
791610 |
ATCAATGGAGGTCAACGGCGAC |
CAGCATGCGTGAAGCCGCCC |
62.5 |
|
fabp1b.1 |
554095 |
GAACTAACGTGTGCTGCTTGTG |
CACGTTTGCTGATGCGCTTGTA |
57.0 |
|
fabp7a |
58128 |
CCATCCATCAGATTTCTATGTGGG |
CATTATGCCTTCTCGTATGTGCG |
56.5 |
|
fabp7b |
407736 |
TTGGAAATGTGACCAAACCGACGC |
TCGTCTCGAAAGGGAATGCAGTGT |
61.5 |
|
fabp10a |
171481 |
TCCAGCAGAACGGCAGCGAC |
CGCCTGTAAAGTGAAGCCATTTCCA |
61.0 |
|
fabp11a |
447944 |
CCAAGCCGTTTTTGATGATGTGAG |
GCTATTAATTTCCCATCCGACACC |
57.0 |
|
acox1 |
449662 |
GGCTACTCCCGCTGCAGCAG |
GGCCTGAGGGTTGTTGGGCC |
63.0 |
| rpl13α | 560828 | ACCAACCCTTCCCGTGGACCA | AGCCAATGCTTGCTTCTACAACAGA | 61.5 |
aAT, annealing temperature (°C).