Figure 4. P. chabaudi infection induces IFNB production in pDCs and RPMs.

(A) No splenocytes are YFP⁺ in mock-infected samples, but some splenocytes become YFP⁺24 h after P. chabaudi infection of C57BL/6 Ifnb reporter mice. 2.5×10⁶ events are depicted in each dot plot. (B) pDCs and RPMs constitute over 90% of YFP⁺ events in C57BL/6 mice. (C) Both pDCs and RPMs contribute to splenic IFNB production in 129Sv mice. pDCs were depleted with a single 500 µg dose of anti-mPDCA1 antibody 18 h before infection with P. chabaudi. Grey dots represent individual mice, with horizontal bars representing the mean (B) or geometric mean (C).