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. 2012 Oct 29;7(10):e38388. doi: 10.1371/journal.pone.0038388

Figure 6. VEGF derived ROS stabilize HIF-1α protein.

Figure 6

(A) Hs29-4T cells were serum-starved for 24 h and then incubated under normoxia or hypoxia in presence of recombinant VEGF 30 ng/ml from 0 to 30 min. Hydrogen peroxide production was evaluated with DCDF-DA and normalized on protein content. Cells were serum-starved for 24 h and incubated under normoxia or hypoxia for 24 h in the following different conditions: (B) neutralizing antibody for VEGF; (C) increasing concentrations of bevacizumab; (D) neutralizing antibody for KDR and Flt-1 receptors. HIF-1α expression was revealed by immunoblot. β-Actin is shown as loading control. *P<0,001 in A versus untreated control under hypoxia at 15 min.