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. 2012 Oct 29;7(10):e38388. doi: 10.1371/journal.pone.0038388

Figure 7. Bevacizumab inhibits ROS production under hypoxia.

Figure 7

(A, B) Hs29-4T cells were serum-starved for 24 h. Cells were then incubated under normoxia or hypoxia of increasing concentrations of bevacizumab (Bev) for 4 and 24 h. Hydrogen peroxide production was evaluated with DCDF-DA and normalized on protein content. *P<0,001 versus untreated control under hypoxia. (C, D) HIF-1α expression was assessed by Western blotting in Hs29-4T cells serum-starved for 4 and 24 h and incubated under normoxia or hypoxia in the absence or presence of DPI (5 µM) and rotenone (1 µM) for additional 24 h. (E) HIF-1α expression was assessed by Western blotting in Hs29-4T cells serum-starved for 24 h and incubated under normoxia or hypoxia in the presence of Apocynin (30 µM) for additional 4 and 24 h.