Figure 3. Tbx5+Gata4+Myocd was the most effective activator of cardiac genes.

10T1/2 fibroblasts were infected with Tbx5, Gata4, Myocd lentivirus alone, or different combinations. LacZ lentivirus was used as control. Genetically unbiased genome-wide expression profiles were obtained 14 days after doxycycline induction. (A) Global gene expression. (B) Cardiac structural gene enrichment. (C) Smooth muscle gene enrichment. (D) Skeletal muscle gene enrichment. (E) Cardiac-related transcription factor enrichment. Signal values were log₂ transformed. Signal values below 0 were set to 1. Each group included biological triplicates. T, Tbx5; G, Gata4; M, Myocd; TG, T+G; GM, G+M; TM, T+M; TGM, T+G+M.