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. 2012 Oct 29;7(10):e48061. doi: 10.1371/journal.pone.0048061

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© 2012 Madhira et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Morphological changes in MSCs from Mutant, Lean and Control rats during adipogenic induction were visualized under phase contrast microscope (Nikon T2000, Japan) at day 1, 7 and 14 demonstrating the formation of lipid-droplet filled cells (arrows). Adipocyte formation was confirmed by staining with Oil Red O (arrows). Magnifications have been represented using scale bar. Panel B shows the quantification for increase in mature adipocytes formed from Mutant as compared to Lean and Control, using the ACT2U soft ware (Nikon, Japan). Values have been represented as Mean ± SE (n = 6), from three independent experiments performed in duplicates. Asteric (*) represents statistical significance (p<0.05) compared to Control as analyzed by ANOVA.