Table 3. Gene Sequences of Primers.
| Group | Gene | Forward Primer | Reverse Primer | Product Size (bp) |
| Embryonic Markers | Oct-4 | cggaagagaaagcggact | gccggttacagaaccaca | 157 |
| Sox2 | cggcaaccagaagaacag | tctcggtctccgacaaaa | 167 | |
| Mesenchymal Markers | Dact-1 | cttgccatctccaagcag | tgccctgtgggacactac | 206 |
| Preadipocyte Markers | Pref-1 | attcgtcgacaagacctgca | ccaccagcctggtgagcacg | 356 |
| Adipogenic Transcriptional Factors | PPARγ2 | tgacagtgacttggccatatt | gcagagggtgaaggctcata | 266 |
| C/EBPα | gccaagaagtcggtggataa | ccttgaccaaggagctctca | 232 | |
| TGFβ1 | gcctccgcatcccacctttg | cgggtgacttctttggcgt | 350 | |
| Mature Adipocyte Markers | Leptin | cctgtggctttggtcctatctg | aggcaagctggtgaggatctg | 244 |
| GLUT-4 | agagtgcctgaaacc | ccctaagtattcaagttctg | 114 | |
| Adiponectin | gtcactgtccccaatgttcc | agaggcctggtccacatttt | 360 | |
| Insulin Signaling Markers | IRS-1 | accacttggaacgtcgtg | tggggtccactctctgtg | 210 |
| Cytokine/Chemokine Markers | IL-6 | cagccagttgccttcttg | tgcatcatcgctgttcat | 219 |
| TNFα | gcaaaccaccaagcagag | cggagaggaggctgactt | 232 | |
| Pancreatic lineage markers | Insulin | caatcatagaccatcagcaagc | tttattcattgcagaggggtgg | 212 |
| PDX-1 | tacaaggacccgtgcgcatt | tcaagttgagcatcactgcc | 451 | |
| GLUT-2 | ccacccagtttacaagctc | tgtaggcagtacgggtcctc | 325 | |
| Housekeeping Gene | β-actin | tgtgatggtgggaatgggtcag | tttgatgtcacgcacgatttcc | 498 |
Abbreviations:
Pref-1: Preadipocyte factor-1, PPARγ2: Peroxisome proliferator activator receptor gamma 2, C/EBPα: CCAAT/enhancer- binding protein alpha, TGFβ1: Transforming growth factor beta 1, AdipoQ: Adiponectin, GLUT-4: Glucose Transporter -4, IRS-1: Insulin receptor substrate -1, IL-6: Interleukin-6, TNFα: Tumor necrosis factor alpha, PDX-1: Pancreatic duodenum homeobox-1, GLUT-2: Glucose Transporter -2.