Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: Arch Biochem Biophys. 2012 Aug 30;528(1):45–49. doi: 10.1016/j.abb.2012.08.007

A, Spectral changes at 458 nm during reduction of PAO by spermine in 200 mM CHES, pH 9.0, 30 °C. (Only 20% of the points are shown for clarity.) The line is from a fit to eq 5. B, Dependence of the flavin reduction constant (k_red) on the spermine concentration for the wild-type enzyme. The line is from a fit of the data to the Michaelis-Menten equation.

A, Spectral changes at 458 nm during reduction of PAO by spermine in 200 mM CHES, pH 9.0, 30 °C. (Only 20% of the points are shown for clarity.) The line is from a fit to eq 5. B, Dependence of the flavin reduction constant (k_red) on the spermine concentration for the wild-type enzyme. The line is from a fit of the data to the Michaelis-Menten equation.