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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Inorg Biochem. 2012 Jun 19;116C:126–134. doi: 10.1016/j.jinorgbio.2012.06.008

Fig. 2. De novo Fe-S cluster assembly on Fdx in the presence of SufB2C2, SufBC2D, or SufA.

Fig. 2

A, 1 mM FAS and Na2S were added to a solution containing 200 μM apo-Fdx with 200 μM (A) apo-SufB2C2, (B) apo-SufBC2D, or (C) apo-SufA and CD spectra were recorded at 5 min intervals for 2 h with a final scan at 18 h. For clarity only spectra obtained at 5, 10, 20, 60, and 120 min are shown. D, Comparison of cluster formation on Fdx in the presence of apo-SufB2C2 (squares), apo-SufBC2D (diamonds), apo-SufA (circles), or just Na2S and FAS (filled triangles). Holo-Fdx formation was calculated by comparing the changes in ellipticity at 434 nm with the 434 nm ellipticity of 100% [2Fe-2S] Fdx prepared separately. Error bars for multiple experiments are shown (but may be obscured by some sybmols).