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. Author manuscript; available in PMC: 2013 Nov 2.
Published in final edited form as: FEBS Lett. 2012 Oct 1;586(21):3825–3830. doi: 10.1016/j.febslet.2012.09.032

Figure 5. SSB mutant F177C inhibits the DinG DNA helicase activity.

Figure 5

A), purified DinG was incubated with the 32P-radioactive-labeled substrate (2 nM) and ATP (2 mM) with or without SSB mutant F177C at 30°C for 15 min. Lane H, the sample was heated at 85°C for 5 min. Lane 1, no DinG. Lanes 2 to 4, with 50, 100 and 200 nM DinG. Lanes 5 to 8, with 1 μM SSB mutant F177C and 0, 50, 100, and 200 nM DinG. B), purified DinG was incubated with the 32P-radioactive-labeled substrate (2 nM) and ATP (2 mM) with or without bacteriophage protein gp32 at 30°C for 15 min. Lane H, the sample was heated at 85°C for 5 min. Lane 1, no DinG. Lanes 2 to 4, with 50, 100 and 200 nM DinG. Lanes 5 to 8, with 500 nM gp32 and 0, 50, 100, and 200 nM DinG. Data are representative of three independent experiments.