Fig. 4.

Recovery of enzymatic activities of lysozyme fibrils after the FEL irradiation or heating. The hydrolytic activities of lysozyme samples were measured using the bacterial cell wall extract, and the relative value (%) of each sample against the native activity was shown. The measurements were performed at least three times, and the reproducible average values were described. The wave numbers of FELs were tuned to amide I (1,620), II (1,535), and III (1,240), or non-amide region (1,100 and 2,000), and the irradiations were performed at 37 °C for 1.5 h. For non-irradiation, the fibrils were dissolved in water and heated at 30–70 °C for 1.5 h. Then, the enzymatic activities were measured similarly with the above method. The activity of fibrils before the treatment was shown as a reference