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. 2012 Nov;22(11):2120–2129. doi: 10.1101/gr.137596.112

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© 2012, Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 3. — Novel candidate driver cancer genes identified by shRNA screening in Ba/F3 viability assay. (A) Ba/F3 cells were transfected with short-hairpin RNAs (shRNA) targeting indicated genes. Empty vector (pGIPZ) and nonspecific shRNA served as the control. Cells were cultured without IL-3 for 4 wk and harvested for viability assay. Cell viability relative to Ba/F3 parental cells was shown. (*) P < 0.05, compared with Ba/F3 parental control. (B) Whole cell lysates were also collected for Western blotting with indicated antibodies, and ERK2 was used as the loading control.

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